CONTROLS FOR DETERMINING REACTION PERFORMANCE IN POLYNUCLEOTIDE SEQUENCE DETECTION ASSAYS

摘要

The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample. In some embodiments of the present teachings, probes are hybridized to complementary target polynucleotides and are ligated together to form a ligation product. Some embodiments of the present teachings comprise positive assay control probes that provide information regarding the occurrence of specific ligation in a complex ligation assay mixture. Some embodiments of the present teachings provide for negative assay control probes that provide information regarding the occurrence of non-specific ligation in a complex ligation assay mixture. Some embodiments of the present teachings provide for the generation of two distinct signals from a monomorphic target polynucleotide sequence.