摘要 |
<p>A method in which 5'-position hydroxy groups are efficiently acylated in the so-called capping step. The method comprises a capping step in which the 5'-hydroxy group of each ribose moiety in an oligonucleic acid derivative represented by the following general formula (2) is protected with an acyl group, and is characterized by using a phenoxyacetic acid derivative anhydride represented by the following general formula (11a) as an acylating agent and a pyridine derivative represented by the following general formula (11b) or (11c) as an activator for the acylation. Thus, an oligonucleic acid derivative represented by the following general formula (12) is produced.</p> |