Acid-labile subunit (ALS) of insulin-like growth factor binding protein complex

摘要

The acid-labile sub-unit (ALS) of insulin like growth factor binding protein complex in biologically pure form is described. ALS has a molecular weight between 80-115 kd as determined by SDS polyacrylamide gel electrophoresis, run under reducing conditions; and a partial N-terminal amino acid sequence as follows: Gly AspProGlyThrProGlyGluAlaGluGlyProAlaCysProAlaAla Cys-Ala wherein the first amino acid may be Gly or Ala. Also described are methods of producing ALS, compositions containing the in-vivo IGF protein complex, methods of detecting ALS in body fluids, recombinant nucleic acid sequences encoding ALS, and expression vectors and host cells containing such nucleic acid sequences.