METHOD, KIT AND INITIATORS FOR GENETIC IDENTIFICATION OF MYCOBACTERIUM BOVIS BCG VARIETY MOREAU-RJ, METHOD FOR MONITORING FEASIBILITY OF BCG MOREAU-RJ AND EXPRESSION OF GENES, AND PRODUCTS WITH IMMUNOLOGICAL ACTIVITY

摘要

This invention refers to the mapping of insertions, deletions and point mutations of BCG Moreau from determining the genomic sequence of BCG Moreau and his comparison with the genome of other mycobacteriae. Moreover, this invention identifies nucleotidic and peptidic sequences, which provide methodologies for genetic verification of BCG Moreau, and for the distinction between BCG Moreau and other mycobacteriae, including in biological and clinical samples. The present invention supplies, furthermore, methods and kits for identification of such sequences, of the products of expression of these sequences or antibodies generated from this expression. The invention furthermore provides methodologies for monitoring and preparation of vaccines and kits for verifying the gene transcription/expression of certain antigens of the bacillus. From this invention are also provided secondary products with immunomodulatory activity, for example, in the treatment of bladder cancer and asthma.