METABOLICALLY OPTIMIZED CELL CULTURE

摘要

Improved methods for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

主权项

1. A method for culturing cells comprising:
(a) culturing cells in a first cell culture; (b) determining a metabolic shift to lactate consumption has occurred in said first cell culture; (c) transferring an amount of cells from said first cell culture to a second cell culture after the metabolic shift to lactate consumption in the cells of said first cell culture has occurred; (d) culturing said cells in a second cell culture; and (e) transferring an amount of cells from said second cell culture to a production cell culture after said cells in the second cell culture have reached a predetermined viable cell concentration.