Allograft tolerance induction

摘要

A method inducing chimerism and allograft tolerance by co-infusion of stem/progenitor-like cells and donor cells, wherein the donor cells can be bone marrow cells. The method also comprises the conditioning comprising depletion of CD4+ and CD8+ T-cells and administration of low doses of anti-neoplastic drugs. The inventive method comprises an aspect wherein allograft tolerance is induced without systemically suppressing the immune system.

主权项

1. A transplantation method comprising inducing allograft tolerance and chimerism by: a) administering, intravenously, subcutaneously, or intradermally, to a host one or more doses of human stem/progenitor-like cells, wherein said human stem/progenitor-like cells are adipose-derived stem cells isolated from fresh human subcutaneous adipose lipoaspirate; b) administering, intravenously, subcutaneously or intradermally, to a host, donor cells, wherein said donor cells are allogeneic to the host, and wherein said donor cells are unprocessed bone marrow, or low density bone marrow cells, wherein said donor cells are administered up to ten (10) days after administration of said human stem/progenitor-like cells in step (a); c) transplanting tissue, allogenic to the host but autologous to the donor cells up to ten (10) days before or after administration of said human stem/progenitor cells; and d) depleting of CD4+ and CD8+ cell of the host; and e) administering a nonmyeloablative anti-neoplastic agent.