System for increasing gene expression and vector comprising the system

摘要

An object of the present invention is to provide a method for increasing the expression of foreign genes, in particular, using a promoter, an enhancer, and the like, and an expression cassette containing a promoter, an enhancer, and the like, by which gene expression can be increased. The purpose is achieved with the use of the gene expression cassette comprising a DNA construct containing a gene to be expressed and a poly A addition sequence that are located downstream of a 1st promoter, and further comprising an enhancer or a 2nd promoter ligated downstream of the DNA construct.

主权项

1. A gene expression cassette, which comprises:
(i) a DNA construct in which a promoter, a gene to be expressed, and a polyA addition sequence are linked in such order; and (ii) enhancer(s) or enhancer(s) with an upstream activation sequence (UAS) ligated to the upstream portion thereof, which comprises at least an hTERT enhancer, in such order, wherein the enhancer(s) or the enhancer(s) with a UAS ligated to the upstream portion thereof is ligated immediately downstream of the polyA addition sequence, and can increase the protein expression from the gene compared to a gene expression cassette in which the enhancer(s) are inserted upstream of the promoter.