| 摘要 |
<p>A method of separating or distinguishing proteins by isoelectric focusing (IEF), comprises fluorescently labelling proteins by reacting monobromobimane or monochlorobimane with free sylphydryl groups of the proteins, and subjecting the labelled proteins to isoelectric focusing. The IEF may be followed by a second electrophoretic separation step, resulting in 2-D electrophoresis of fluorescently pre-labelled proteins in a way that has not hitherto been possible.</p> |
