发明名称 |
Device for monitoring cells |
摘要 |
The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
|
申请公布号 |
US6395506(B1) |
申请公布日期 |
2002.05.28 |
申请号 |
US19990342720 |
申请日期 |
1999.06.29 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
PITNER J. BRUCE;HEMPERLY JOHN JACOB;GUARINO RICHARD D.;WODNICKA MAGDALENA;STITT DAVID T.;BURRELL GREGORY J.;FOLEY, JR. TIMOTHY G.;BEATY PATRICK SHAWN |
分类号 |
C12Q1/04;C12Q1/18;(IPC1-7):C12Q1/18 |
主分类号 |
C12Q1/04 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|