ELECTROPHORETIC TAG-BASED IN VITRO ASSAY TO QUANTIFY DIMERIZATION OF P66 AND P51 SUBUNITS OF HIV-1 REVERSE TRANSCRIPTASE (RT)

摘要

Methods for measuring hetero- and homodimerization of HIV reverse transcriptase (HIV RT) are provided using pairs of tagged probes and cleaving probes. The methods can be used, for example, to screen for modulators of HIV RT dimerization. Also provided are methods of determining whether a compound modulates HIV RT. Further provided are methods of determining whether a compound modulates formation of a complex between a p66 and p51, p66 and p66, or p51 and p55 subunits polypeptides of HIV RT.