发明名称 |
Method of Isolating P450 Gene |
摘要 |
The present invention provides a method for preparing a hybrid gene. The method includes a step of amplifying a P450 gene fragment contained in a sample using primers designed on the basis of regions of a plurality of P450 in which amino acid sequences are highly conserved and a step of preparing the hybrid gene using the amplified fragments and a known P450 gene. The method includes no culturing step or a step of normalizing extracted DNAs and is useful in isolating a P450 gene from various microbial resources. The present invention further provides a fused cytochrome P450 monooxygenase containing a peptide which is linked to the C-terminus of a P450 protein with a linker portion disposed therebetween and which has the same function as that of a reductase domain contained in a cytochrome P450 monooxygenase originating from Rhodococcus sp. strain NCIMB 9784. This enables the construction of a high-efficiency electron transfer system useful for various P450 proteins and also enables the production of an active P450 monooxygenase.
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申请公布号 |
US2008220419(A1) |
申请公布日期 |
2008.09.11 |
申请号 |
US20050665843 |
申请日期 |
2005.11.02 |
申请人 |
MARINE BIOTECHNOLOGY INSTITUTE CO., LTD. |
发明人 |
KUBOTA MITSUTOSHI;NODATE MIHO;NODATE KENICHI;UCHIYAMA TAKU;MISAWA NORIHIKO |
分类号 |
C12Q1/68;C07H1/00;C07H21/00;C07K14/00;C12N1/00;C12N9/04;C12N15/00;C12N15/63;C12P7/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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