TEST APPARATUS AND METHODS FOR ST2 CARDIAC BIOMARKER

摘要

The technology described in this document can be embodied in a test strip for use in measuring a level of an ST2 cardiac biomarker in a whole blood sample. The test strip includes a base, and a plurality of conjugates, wherein each conjugate includes a reporter group bound to a first antibody that binds to ST2. A conjugate pad holds the plurality of conjugates that bind with ST2 to produce conjugate-ST2 complexes. The test strip also includes a plurality of second antibodies that bind to ST2, and a plurality of third antibodies that bind to the conjugate-ST2 complexes. The plurality of second antibodies are bound to a membrane in a test location, and the plurality of third antibodies are bound to the membrane in a control location. A plasma separation pad passes blood plasma from a whole blood sample to the conjugate pad while inhibiting other components.

主权项

1. A test strip for use in measuring a level of an ST2 cardiac biomarker in a whole blood sample, the test strip comprising:
a base; a plurality of conjugates, each conjugate comprising a reporter group bound to a first antibody that binds to ST2; a conjugate pad disposed along a length of the base and configured to hold the plurality of conjugates that bind with ST2 to produce conjugate-ST2 complexes; a plurality of second antibodies that bind to ST2; a plurality of third antibodies that bind to the conjugate-ST2 complexes; a membrane disposed on the base such that the membrane is in fluid communication with the conjugate pad, and wherein the plurality of second antibodies are bound to the membrane in a test location and the plurality of third antibodies are bound to the membrane in a control location arranged further from the conjugate pad than the test location; and a plasma separation pad in fluid communication with a portion of the conjugate pad, wherein the plasma separation pad is disposed and configured to receive the whole blood sample and to pass blood plasma from the whole blood sample to the conjugate pad while inhibiting other components of the whole blood sample from passing to the conjugate pad.