| 摘要 |
The present invention relates to a production method of a TLR5 agonist protein. According to the present invention, the TLR5 agonist protein can be produced through bioengineering, and can be easily separated and purified. Particularly, by effectively removing a fusion partner used for the separation and purification, the production method can minimize possibilities for inducing an immune reaction caused by the fusion partner, and degrading bonding with TLR5. In the present invention, the TLR5 agonist protein is produced by: producing a vector including a fusion gene which is obtained by bonding a gene encoding ubiquitin with a gene encoding a TLR5 agonist protein, formed by bonding polypeptides having an amino sequence described by SEQ ID NO: 4 and polypeptides having an amino sequence described by SEQ ID NO: 2, and by bonding a gene encoding a purifying tag to the gene encoding ubiquitin; transforming a host using the vector, and expressing the fusion gene to obtain a fusion protein; bonding the fusion protein to a column where the purifying tag is bonding to obtain the fusion protein; and obtaining the TLR5 agonist protein after cutting the bonding site of ubiquitin. |
