| 摘要 |
Detection of cytosine methylation in DNA and detecting to what extent the (A)-treated and original DNAs differ, and deducing the methylation status at least one locus in the DNA, is new. Detection of cytosine methylation in DNA comprises: (i) incubating a genomic DNA sample with 0.1-6 M bisulfite solution (A); (ii) dilution with water or aqueous solution; (iii) amplification in a polymerase reaction; and (iv) detecting to what extent the (A)-treated and original DNAs differ, and deducing the methylation status at least one locus in the DNA. Step (i) is in presence of a denaturing agent and/or solvent, also of at least one radical scavenger (I). An independent claim is also included for a kit for the process comprising (A), denaturant or solvent, (I), primers for amplification and instructions. |
