Marker and reagent for detection of human IL-17-producing helper T cells, and method for detection of human IL-17-producing helper T cells

摘要

The present invention relates to a marker allowing specific detection of human IL-17-producing helper T-cells (human Th17 cells), a method for specifically detecting human Th17 cells and a reagent for detecting human Th17 cells.

主权项

1. A method for detecting human IL-17-producing helper T-cells comprising:
isolating CD4-positive cells from a blood sample with an anti-CD4 antibody; mixing at least some of said CD4-positive cells with a labeling substance and with an antibody that specifically binds to a protein encoded by a PTPRM gene, wherein one or more of the CD4-positive cells, the antibody that specifically binds to a protein encoded by a PTPRM gene, and the labeling substance, form a complex; applying said complex to a flow cytometer; and detecting the presence of an IL-17-producing helper T-cell by detecting the label of the complex using the flow cytometer, wherein said sample is determined to contain a human IL-17-producing helper T-cell when the label is detected with the flow cytometer in the detecting step.