发明名称 |
Template switch-based methods for producing a product nucleic acid |
摘要 |
Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits. |
申请公布号 |
US9410173(B2) |
申请公布日期 |
2016.08.09 |
申请号 |
US201314058038 |
申请日期 |
2013.10.18 |
申请人 |
Clontech Laboratories, Inc. |
发明人 |
Betts Craig;Farmer Andrew Alan |
分类号 |
C12P19/34;C12N15/10 |
主分类号 |
C12P19/34 |
代理机构 |
Bozicevic, Field & Francis LLP |
代理人 |
Field Bret E.;Bozicevic, Field & Francis LLP |
主权项 |
1. A method comprising:
combining:
a template deoxyribonucleic acid (DNA);a retroviral reverse transcriptase;a template switch oligonucleotide;a first DNA primer comprising a 3′ domain that hybridizes to the template DNA; anddNTPs; into a reaction mixture under conditions sufficient to produce a product nucleic acid, wherein the product nucleic acid comprises the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single hybrid nucleic acid comprising a region polymerized from the dNTPs by the retroviral reverse transcriptase, wherein the region polymerized from the dNTPs comprises a domain that is complementary to and hybridized to the 5′ end of the template DNA. |
地址 |
Mountain View CA US |