NOVEL GLUCOSE DEHYDROGENASE

摘要

The invention relates to a glucose dehydrogenase showing an NADP/NAD activity ratio, namely the value obtained by dividing the enzyme activity value obtained by using NADP as a coenzyme by the enzyme activity value obtained by using NAD as a coenzyme, of not lower than 300, to a gene coding therefor, and to a transformant harboring that gene. The enzyme of the invention is very high in NADP specificity and can be suitably used, for example, for NADPH production, for coenzyme regeneration in enzymatic reduction reactions, and in biosensors for glucose concentration measurements.

主权项

1. A DNA coding for a NADP-specific glucose dehydrogenase showing a NADP/NAD activity ratio of no lower than 300, derived from Lactobacillus plantarum, which is selected from the group consisting of:
(a) a DNA having the base sequence of SEQ ID NO:2; (b) a DNA coding for a polypeptide having the amino acid sequence of SEQ ID NO:1; (c) a DNA hybridizing with a DNA having a base sequence complementary to the base sequence of SEQ ID NO:2 under stringent conditions and coding for a polypeptide acting on glucose and NADP to form gluconolactone and NADPH; and (d) a DNA having a sequence identity of at least 90% with the base sequence of SEQ ID NO:2 and coding for a polypeptide acting on glucose and NADP to form gluconolactone and NADPH.