THERMOSTABLE TAQ POLYMERASE FRAGMENT

摘要

It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq.DELTA.288) possesses an increased thermostability over TaqWT, Taq.DELTA.279, and Taq.DELTA.289. The present invention therefore provides Taq.DELTA.288, recombinant expression vectors encoding th e same or derivatives thereof, as well as purification protocols for Taq.DELTA.288. Th e invention also encompasses kits containing Taq.DELTA.288 as well as the use of Taq.DELTA.288 and kits containing Taq.DELTA.288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucle ic acid.