METHOD FOR DETECTING MUTATION OF CYP2D6 AND NUCLEIC ACID PROBE AND KIT THEREFOR

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting a mutation at the 100th position (C100T mutation) of a cDNA of a CYP2D6 gene.

SOLUTION: A nucleic acid probe obtained by amplifying a region containing the C100T mutation according to a PCR (polymerase chain reaction), labeling the terminus with a fluorescent dye, reducing the fluorescence of the fluorescent dye when hybridized and having a specific base sequence is used to carry out a melting curve analysis by measuring the fluorescence of the fluorescent dye. The mutation is detected on the basis of the results of the melting curve analysis.

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