Enzymatic method for producing dihydroxyacetone phosphate

摘要

A method for making dihydroxyacetone phosphate (DHAP) comprising treating dihydroxyacetone with a bacterial acid phosphatase in the presence of pyrophosphate. The invention further pertains to a method for making, preferably in one pot, stereospecifically a compound of the formula:
€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ R-C*H(OH)-C*H(OH)-CO-CH 2 -OH
comprising the steps:
a) treating dihydroxyacetone with a bacterial acid phosphatase in the presence of pyrophosphate to make dihydroxyacetone phosphate;
b) treating the dihydroxyacetone phosphate with R-CHO and an aldolase, wherein R is an alkyl group and C* stands for a chiral carbon atom to a phosphate of the formula:
€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ R-C*H(OH)-C*H(OH)-CO-CH 2 -OPO 3 H 2 ; and
c) dephosphorylating the phosphate of step b) by treating said phosphate with a bacterial acid phosphatase.