A PROCESS FOR ISOLATION AND PURIFICATION OF A TARGET PROTEIN FREE OF PRION PROTEIN (PRPSC)

摘要

A process for isolation and purification of a target protein by chromatography wherein the chromatography removes or depletes prions (PrPsc ), comprising the steps of - contacting a potentially PrPsc-contaminated sample comprising a target protein with a multimodal chromatographic material; - setting buffer conditions so that the target protein is bound to the multimodal chromatographic material and whereas PrPsc is not binding to the multimodal chromatographic material; - followed by elution of the target protein.