Method for monitoring cell viability

摘要

A method for measuring through optical means temporally resolved, optical properties, and/or phenotypes that are linked to cellular homeostasis. Those temporal measurements enable the detection of cell regulation through various channels linked to homeostasis, in order to monitor cell viability.

主权项

1. A method for monitoring cell viability in a sample, said method comprising the following steps:
interacting an electromagnetic wave with at least one cell of said sample; measuring at least one optical property of said cell, said at least one optical property being probed with said electromagnetic wave and said at least one optical property being the phase of said electromagnetic wave interacting with said at least one cell; carrying out a cell environment perturbation of said at least one cell; making temporally resolved measurements of said optical property with digital holographic microscopy to monitor a cell regulation behaviour of said at least one cell, said cell regulation behaviour being a cell mechanism activated to restore a homeostatic equilibrium of said at least one cell after said cell environment perturbation; and determining said at least one cell is viable when a change in the measured optical property, due to said cell environment perturbation, is reversible such that the measured optical property recovers to a level comparable to that before said cell environment perturbation which indicates a restoration of cell homeostatic equilibrium by cell regulation.