| 主权项 |
1. An isolated polynucleotide encoding an endoglucanase polypeptide variant comprising an amino acid sequence that is at least 90% identical to the endoglucanase polypeptide of SEQ ID NO:2, comprising a substitution at position 166 and having at least one additional substitution of an amino acid residue at a position V2, D3, D8, G14, Y18, M20, Y21, V25, F42, A57, D58, 161, N62, S90, H96, V97, D104, D109, M114, G122, N146, E148, K153, L173, D175, D180, A185, K204, L206, D214, T224, A226, E231, K235, V236, I238, V243, T254, D256, W270, K283, P286, 1287, D288, L292, S294, 1296, V297, M319, Y328, D329, I342, L343, H350, Q357, T361, L362, V368, Q369, R371, H373, L390, N392, E399, A403, K416, 1418, K423, P425, K429, and/or L433, wherein amino acid position is determined by alignment with SEQ ID NO:2. |
