发明名称 |
Method for isolating and purifying bordetella pertussis antigenic factors |
摘要 |
A process is provided for the purification of pertussis toxin (PT) and/or filamentous hemagglutinin antigen (FHA) from a B. pertussis fermentation broth or cell free culture supernatant containing at least one antigen, which comprises contacting the fermenation broth or cell free culture supernatant with a hydroxyapatite adsorbent for a sufficient time to adsorb the antigen(s) at a pH which both PT and FHA are adsorbed and eluting a mixture containing the adsorbed antigen(s) from the adsorbent with eluant at a pH which both PT and FHA are eluted. The PT and FHA may be further purified by two sequential chromatographic columns, one of which involves apolar-ligand chromatography.
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申请公布号 |
US5391715(A) |
申请公布日期 |
1995.02.21 |
申请号 |
US19920856817 |
申请日期 |
1992.03.24 |
申请人 |
SMITHKLINE BEECHAM BIOLOGICALS |
发明人 |
CAPIAU, CARINE;DESMONS, PIERRE |
分类号 |
A61K39/00;C07K14/195;(IPC1-7):C07K3/02;C07K3/20;C07K15/04 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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