METHOD FOR SCREENING ANTI-LIGAND LIBRARIES FOR IDENTIFYING ANTI-LIGANDS SPECIFIC FOR DIFFERENTIALLY AND INFREQUENTLY EXPRESSED LIGANDS

摘要

The present invention relates to screening methods and, in particular, to methods of screening anti-ligand libraries for identifying anti-ligands specific for differentially and/or infrequently expressed ligands. The method comprises the steps of providing a library of anti-ligands; providing a first subtractor ligand; providing a second target ligand; determining the amount of the first and second target ligands using one or more equations derived from the universal law of mass action; providing the determined amount of a first subtractor ligand; providing the determined amount of a second target ligand: providing separation means capable of use to isolate anti-ligand bound to the second target ligand from anti-ligand bound to the first subtractor ligand; exposing the library of to the first and second target ligands to permit binding of anti-ligands to ligands; and using the separation means to isolate the anti-ligand bound to second target ligand.

主权项

1. A method of isolating at least one anti-ligand to at least one differentially expressed target ligand comprising the steps of:
(i) providing data set(s) describing a library of anti-ligands; (ii) providing data set(s) describing a population of subtractor ligand constructs, said subtractor ligand constructs comprising a ligand associated with a separation means, wherein said separation means is a physical feature of the subtractor ligand construct; (iii) providing data set(s) describing a population of target ligand constructs, said target ligand constructs comprising the same ligand as step (ii) associated with a separation means, wherein said separation means is a physical feature of the target ligand construct; (iv) determining the amounts of subtractor ligand construct and target ligand construct utilizing one or more equations derived from the universal law of mass action[C]c[D]d[A]a[B]b=Keq,where:
A, B, C & D=are the participants in the reaction (reactants and products) a, b, c, & d=the coefficients necessary for a balanced chemical equation to permit the determination of the amount of subtractor ligand as compared to target ligand required for isolation of at least one anti-ligand to at least one target ligand; (v) providing the amount of the subtractor ligand construct determined in step (iv); (vi) providing the amount of the target ligand construct determined in step (iv); (vii) providing the library of anti-ligands described by the data-set of step (i) (viii) exposing the anti-ligand library of (vii) to the amounts of subtractor ligand construct provided in step (v) and the amounts of the target ligand construct provided in step (vi) to permit binding of anti-ligands to ligands; and (ix) isolating the anti-ligand bound to the target ligand construct.