| 主权项 |
1. A method for determining tissue oxygenation comprising:
at a system comprising a processor and memory, the memory including instructions for: obtaining a data set comprising a plurality of images of a tissue of interest, each respective image in the plurality of images resolved at a different spectral band, in a predetermined set of eight to twelve spectral bands, and comprising an array of pixel values; registering, using the processor, the plurality of images on a pixel-by-pixel basis, to form a plurality of registered images of the tissue; and performing spectral analysis at a plurality of points in a two-dimensional area of the plurality of registered images of the tissue, the spectral analysis including determining approximate values of oxyhemoglobin levels and deoxyhemoglobin levels at each respective point in the plurality of points, wherein the predetermined set of eight to twelve spectral bands includes spectral bands having central wavelengths of:
(i) 510±3 nm, 530±3 nm, 540±3 nm, 560±3 nm, 580±3 nm, 590±3 nm, 620±3 nm, and 660±3 nm,(ii) 520±3 nm, 540±3 nm, 560±3 nm, 580±3 nm, 590±3 nm, 610±3 nm, 620±3 nm, and 640±3 nm, or(iii) 500±3 nm, 530±3 nm, 545±3 nm, 570±3 nm, 585±3 nm, 600±3 nm, 615±3 nm, and 640±3 nm; and wherein each respective spectral band in the eight to twelve spectral bands has a full width at half maximum of less than 15 nm. |
