| 摘要 |
A method, reagent mixture and test kit are provided for testing a urine sample for the presence of bacterial or somatic cells, by reacting a urine sample with a catalase-free alkaline protease enzyme and a detergent compatible therewith, so as to disrupt any such cells present in the sample and release active catalase therefrom. The presence of catalase is detected by the formation of foam upon the addition of H2O2. The reaction of the sample with the protease enzyme and the detergent is conducted in the presence of: (a) a buffer providing for a pH of about 8.5 to about 9; and optionally (b) one or more additional solutes; the total concentration of components (a) and (b), when present, being from about 0.02M to about 0.4M.
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