发明名称 |
The use of DNA polymerase having 3'-intrinsic editing activity |
摘要 |
The invention is directed to the use of RNA or DNA polymerases having 3'-intrinsic editing activity (3'-IEA) in the presence or absence of a deactivating agent to remove a protecting group from the 3'-position of oligo- or polyribo- or deoxyribonnucleotides. The use is concerned with the incorporation of dNTPs into DNA templates in order to determine the concentration and / or sequence of said templates. In particular the use is concerned with an improved non gel-based sequencing method- <IMAGE>
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申请公布号 |
EP0745688(A1) |
申请公布日期 |
1996.12.04 |
申请号 |
EP19960108543 |
申请日期 |
1996.05.29 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
CANARD, BRUNO;SAGNER, GREGOR, DR. |
分类号 |
C12Q1/48;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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