COMPOSITE MEMBRANE TO CAPTURE ANALYTE TRANSFERS FROM GELS

摘要

Proteins and other analytes that have been separated by electrophoretic means in a gel are transferred to a membrane by conventional blotting techniques, the membrane being a composite of an analyte binding layer and a size retention layer. The pore size of the size retention layer is large enough to allow non-macromolecular ions to pass, yet not large enough to allow the passage of the analytes from the gel, nor antibodies or other macromolecules or reagents in general that might be used in the detection, imaging, or quantification of the analytes.