TARGET NUCLEIC ACID DETECTION METHOD, ASSAY KIT, AND PROBE-IMMOBILIZED SUBSTRATE

摘要

A target nucleic acid detection method according to an embodiment comprises: (A) placing a reaction field which contains a sample that is suspected to contain a target nucleic acid, a nucleic acid probe, a coated nucleic acid strand that is bound to the nucleic acid probe, a labeling substance and a primer set under isothermal amplification reaction conditions; (B) monitoring a signal coming from the nucleic acid probe under isothermal amplification reaction conditions; and (C) detecting the target nucleic acid on the basis of the signal obtained in step (B). The nucleic acid probe is immobilized on at least one surface of a substrate. Each of the nucleotide sequence for the nucleic acid probe and the nucleotide sequence for the coated nucleic acid strand is a sequence whereby the competition between an amplification product and the nucleic acid probe, the detachment of the coated nucleic acid strand from the nucleic acid probe and the binding between the coated nucleic acid strand and the amplification product can be achieved under isothermal amplification reaction conditions sequence, or a sequence whereby the binding between the coated nucleic acid strand and the amplification produce and the elongation of the coated nucleic acid strand can be achieved under isothermal amplification reaction conditions while keeping such a state that the nucleic acid probe is bound to the coated nucleic acid strand. The detection of the signal can be inhibited by the presence of a nucleic acid that is bound to the nucleic acid probe or the increase in the amount of the nucleic acid.