Influenza virus-like particles (VLPS) comprising hemagglutinin produced within a plant

摘要

A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

主权项

1. A method of producing influenza virus like particles (VLPs) in a plant, the VLPs comprising protein, wherein the protein consists of influenza hemagglutinin (HA), comprising:
a) introducing a nucleic acid comprising a nucleotide sequence encoding the hemagglutinin (HA) operatively linked to a regulatory region active in the plant into the plant, or portion thereof, b) incubating the plant under conditions that permit the expression of the nucleic acid, thereby producing the VLPs comprising protein, wherein the protein consists of influenza hemagglutinin (HA), c) harvesting the plant, and d) purifying the VLPs, wherein the VLPs range in size from 80-300 nm.