Recombinant <i>Lactococcus lactis </i>expressing <i>Escherichia coli </i>colonization factor antigen I (CFA/I) fimbriae and their methods of use

摘要

The present disclosure relates generally to therapeutic compositions comprising recombinant bacteria. Further, the disclosure elaborates upon methods of utilizing the taught therapeutic compositions to treat autoimmune and inflammatory disease. The present teachings also relate to the disclosed recombinant bacteria and methods of producing the recombinant bacteria utilized in the compositions and methods. Further taught herein are dietary supplements and food additive compositions comprising the taught recombinant bacteria.

主权项

1. A composition for the treatment of an autoimmune or inflammatory disease, the composition comprising:
a recombinant Lactococcus lactis bacterial cell comprising a nucleotide sequence coding for enterotoxigenic Escherichia coli colonization factor antigen I fimbriae genes cfaA, cfaB, cfaC, and cfaE, wherein the bacterial cell expresses E. coli colonization factor antigen 1 fimbriae genes cfaA, cfaB, cfaC, and cfaE; and wherein the cfaA gene comprises SEQ ID NO: 3, the cfaB gene comprises SEQ ID NO: 2, the cfaC gene comprises SEQ ID NO: 4, and the cfaE gene comprises SEQ ID NO: 5, and an acceptable carrier.