C.FIMI GLYCOSYL HYDROLASE CONTAINING A MANNAN BINDING DOMAIN

摘要

Novel polypeptide compositions and methods for their use are provided comprising fusion proteins in which the mannan binding domain or functional portion thereof is fused to a heterologous protein or is conjugated to a chemical moiety. The compositions can be synthesized or prepared by recombinant DNA technology. The compositions find use for separating and/or purifying biological compositions and living cells. In the method for purifying biological compositions, the biological composition is mixed with a phase-forming mannan to which the mannan binding peptide binds inducing phase separation. The phase containing the biological composition is then isolated. Phase separation is induced using the phase separation inducing agent or, when the oligosaccharide polymer is a thermo-separating polymer, by increasing the temperature above the cloud point temperature of the polymer.