A PRIMER FOR DETECTING FOOD BORNE PATHOGENIC MICRO-ORGANISM AND A METHOD FOR DETECTING FOOD BORNE PATHOGENIC MICRO-ORGANISM USING THE SAME

摘要

A primer is provided to amplify a gene specific to Vibrio parahaemolyticus, thereby being used to detect the Vibrio parahaemolyticus infection rapidly, accurately and efficiently through various techniques such as a real time polymerase chain reaction(PCR). A primer for detecting Vibrio parahaemolyticus comprises at least one primer pair selected from the group consisting of a primer pair consisting of a primer of SEQ ID : NO. 7 and a primer of SEQ ID : NO. 8, a primer pair consisting of the primer of SEQ ID : NO. 7 and a primer of SEQ ID : NO. 28, a primer pair consisting of the primer of SEQ ID : NO. 7 and a primer of SEQ ID : NO. 29, and a primer pair consisting of the primer of SEQ ID : NO. 7 and a primer of SEQ ID : NO. 31. A method for detecting the Vibrio parahaemolyticus comprises a step of detecting the Vibrio parahaemolyticus through PCR using the primer for detecting the Vibrio parahaemolyticus, wherein the PCR is multiplex PCR. A kit for detecting the Vibrio parahaemolyticus comprises the primer for detecting the Vibrio parahaemolyticus, a reaction buffer solution, dNTP and Taq DNA polymerase.