发明名称 |
Human enbryonic germ cell line and methods of use |
摘要 |
Primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a 8-11 week LMP human embryo, are disclosed. The primordial germ cells are cultured in long term culture (more than 30 days) resulting in cells that resemble embryonic stem cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
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申请公布号 |
US6331406(B1) |
申请公布日期 |
2001.12.18 |
申请号 |
US19970989744 |
申请日期 |
1997.12.12 |
申请人 |
THE JOHN HOPKINS UNIVERSITY SCHOOL OF MEDICINE |
发明人 |
GEARHART JOHN D.;SHAMBLOTT MICHAEL JOSEPH |
分类号 |
C12N15/09;A61K35/12;A61K48/00;A61P43/00;C12N5/02;C12N5/073;C12N5/074;C12N5/10;C12Q1/02;G01N33/569;G01N33/68;(IPC1-7):G01N33/567;C12N5/08;C12N5/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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