METHOD FOR DETERMINING DEVELOPMENTAL POTENTIAL OF ONE OR MORE HUMAN EMBRYOS TO REACH BLASTOCYST STAGE

摘要

The invention provides a method for determining the developmental potential of one or more human embryos to reach the blastocyst stage, comprising culturing one or more human embryos under conditions sufficient for embryo development; time-lapse microscopy in one or more embryos of a duration of at least one cytokinesis event or cell cycle; measuring at least one cell parameter comprising (a) the duration of the first cytokinesis; or (b) time between the first and second mitosis; or (c) time between the second and third mitosis; and identifying embryo that has a potential to reach the blastocyst stage, when said at least one cell parameter for said embryo corresponds to (i) the duration of the first cytokinesis that is up to 33 minutes; or (ii) time between the first and second mitosis that is about 7.8 to 14.3 hours; or (iii) time between the second and third mitosis 3 that is up to 5.8 hours.