Method for producing methacrylyl-CoA

摘要

The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, 3-hydroxyisobutyryl-CoA is converted into methacrylyl-CoA of the enzyme having dehydratase activity at a rate of 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genus Pseudomonas or Rhodococcus.