发明名称 |
MODIFIED CARBOXYPEPTIDASE |
摘要 |
A method for transamidating a peptide substrate having a P1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S1', S2 and/or S3 subsite to accommodate a specific peptide substrate.
|
申请公布号 |
CA2281879(A1) |
申请公布日期 |
1998.09.03 |
申请号 |
CA19982281879 |
申请日期 |
1998.02.27 |
申请人 |
CARLSBERG LABORATORY |
发明人 |
BREDDAM, KLAUS;SORENSEN, STEEN B.;OLESEN, KJELD;STENNICKE, HENNING;MORTENSEN, UFFE |
分类号 |
C12N9/48;C12P13/02;(IPC1-7):C12N9/48;C12P21/00 |
主分类号 |
C12N9/48 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|