摘要 |
<p>PURPOSE:To efficiently obtain the titled ester, having a high content of unsaturated double bonds and useful as a substitute raw material for sperm whale oil at a low cost, by cultivating a microorganism capable of assimilating an n-paraffin in a culture medium containing a fat or oil. CONSTITUTION:(A) A microorganism of the genus Acinetobacter, Saccharomyces, etc., capable of assimilating an n-paraffin is inoculated into (B) a culture medium consisting of (a) a nitrogen compound, e.g. ammonium sulfate, etc., assimilable by the microorganism, (b) an inorganic salt, e.g. sodium phosphate, ferrous sulfate, etc., (c), as necessary, an organism growth promoting substance, e.g. vitamin, (d) a fat or oil consisting essentially of glycerol ester of an unsaturated fatty acid as a carbon source and a lower organic acid, e.g. acetic acid, etc., as the second carbon source, and a hydrocarbon, e.g. glucose, etc., and aerobically cultivated at 20-40 deg.C while shaking to afford the titled ester.</p> |