发明名称 |
Method for directional stable transformation of eukaryotic cells |
摘要 |
The present invention is drawn to compositions and methods for introducing nucleotide sequences at preferred genomic target sites in a eukaryotic genome. The compositions comprise transfer cassettes which are flanked by nonhomologous recombination sites. The method involves transforming eukaryotic cells containing target sites utilizing non-integrating transformation methods. The method results in efficient integration of nucleotides into predetermined genetic locations and eliminates random DNA integration. |
申请公布号 |
US6331661(B1) |
申请公布日期 |
2001.12.18 |
申请号 |
US19980193484 |
申请日期 |
1998.11.17 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
BASZCZYNSKI CHRISTOPHER L.;LYZNIK LESZEK ALEXANDER;GORDON-KAMM WILLIAM J. |
分类号 |
C12N9/00;C12N15/10;C12N15/113;C12N15/29;C12N15/31;C12N15/63;C12N15/82;C12N15/90;(IPC1-7):C12N15/90;A01H5/00;A01H5/10;C12N5/04 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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