发明名称 |
Envelope gene-deficient paramyxovirus vector |
摘要 |
F gene-deficient virus virions are successfully recovered by using an f gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.
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申请公布号 |
US2002169306(A1) |
申请公布日期 |
2002.11.14 |
申请号 |
US20010966277 |
申请日期 |
2001.09.27 |
申请人 |
KITAZATO KAIO;SHU TSUGUMINE;KUMA HIDEKAZU;UEDA YASUJI;ASAKAWA MAKOTO;HASEGAWA MAMORU;IIDA AKIHIRO;TOKITOU FUMINO;HIRATA TAKAHIRO;TOKUSUMI TSUYOSHI;INOUE MAKOTO |
发明人 |
KITAZATO KAIO;SHU TSUGUMINE;KUMA HIDEKAZU;UEDA YASUJI;ASAKAWA MAKOTO;HASEGAWA MAMORU;IIDA AKIHIRO;TOKITOU FUMINO;HIRATA TAKAHIRO;TOKUSUMI TSUYOSHI;INOUE MAKOTO |
分类号 |
A61K48/00;C12N7/04;C12N15/86;(IPC1-7):C12N15/86;C07H21/04;C12N7/00;C12Q1/70;A61K39/12;A61K39/155;C12N7/01;C12N15/00;C12N15/09;C12N15/63;C12N15/70;C12N15/74 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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