Diversification of human milk oligosaccharides (HMOs) or precursors thereof

摘要

A method of diversification of human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprises a) providing at least one compound or a mixture of the compounds selected from the group consisting of: optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof; b) adding at least one enzyme comprising a transglycosidase activity to the at least one compound or a mixture of compounds provided according to step a); and c) incubating the mixture obtained according to step b).

主权项

1. A method for preparation of two or more human milk oligosaccharides (HMOs) or derivatives or precursors thereof, the method comprising the steps of
a) providing at least one compound or a mixture of the compounds selected from the group consisting of:
optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof: whereinR is a group removable by hydrogenolysis,R1 independently of each other is fucosyl or HR4 independently of each other is sialyl or H,provided that the compound of general formula 2 is not R-glycoside of lactose, if provided alone;optionally sialylated and/or fucosylated lactose derivatives of general formula 4 and salts thereof: whereinR1 independently of each other is fucosyl or HR4 independently of each other is sialyl or H,provided that the compound of general formula 4 is not lactose, if provided alone;lacto-N-tetraose (LNT): lacto-N-tetraose (LNT) derivatives of the following formula: wherein R is a group removable by hydrogenolysis;lacto-N-neotetraose (LNnT): lacto-N-neotetraose (LNnT) derivatives of the following formula: wherein R is a group removable by hydrogenolysis; b) adding at least one enzyme comprising a transglycosidase activity to the at least one compound or a mixture of compounds provided according to step a); c) incubating the mixture obtained according to step b); d) optionally repeating at least steps a) and c) or steps b) and c) with the mixture obtained according to step c); e) optionally subjecting the mixture obtained after step c) or d) to a hydrogenolysis reaction; wherein the incubation results in the production of two or more human milk oligosaccharides or derivatives or precursors thereof.