Method for rejuvenating cells

摘要

The invention relates to a method for reprogramming cells from aged donors or senescent cells to pluripotent cells that have lost marks of senescence. In particular, the invention relates to an ex vivo method for preparing induced pluripotent stem cells (iPSCs) from a target cell population comprising cells from aged donors or senescent cells, said method comprising the steps of culturing said target cell population under appropriate conditions for reprogramming said cells into iPSCs, wherein said appropriate conditions comprises increasing expression in said target cells, of at least the following reprogramming factors: Oct4, Klf4, Sox2, c-Myc, Lin28 and, optionally Nanog.

主权项

1. An ex vivo method for preparing rejuvenated induced pluripotent stem cells (iPSCs) from senescent cells, said method comprising the steps of:
a) providing said senescent cells and, b) culturing said target cell population under appropriate conditions for reprogramming said target cell population into iPSCs, wherein said appropriate conditions comprise increasing expression in said target cell population, of at least the following combination of reprogramming factors:
i. a reprogramming factor encoded by one gene of the Oct gene family,ii. a reprogramming factor encoded by one gene of the Klf gene family,iii. a reprogramming factor encoded by one gene of the Sox gene family, iv. a reprogramming factor encoded by one gene of the Myc gene family,v. Lin28,vi. and Nanog,wherein the method results in preparation of rejuvenated iPSC cells in which the expression of p21CIPI and p16IN1K4A is downregulated to a level found in human embryonic stem cells (hESC).