Polynucleotides encoding rodent antibodies with human idiotypes and animals comprising same

摘要

The invention relates to polynucleotides, particularly chimeric polynucleotides useful for optimal production of functional immunoglobulins with human idiotypes in rodents. The invention further relates to rodents comprising such polynucleotides.

主权项

1. A transgenic rodent, the rodent comprising in its germline a heavy chain immunoglobulin locus comprising human unrearranged heavy chain variable region gene segments linked to DNA encoding a rodent heavy chain constant region and a rat 3′ enhancer comprising the sequence set forth as SEQ ID NO:1, wherein the heavy chain immunoglobulin locus does not comprise a human immunoglobulin constant region gene, and wherein the rodent is a mouse or a rat.