OBTAINING OF CLOSTRIDIUM BACTERIUM MUTANT, SAME MUTANT AND PRODUCTION OF BUTANOL USING THEREOF

摘要

NEW MATERIAL:A Clostridium pasteurianum CA 101 stock (FERM P-10817) as a mutant of genus Clostridium bacterium having analog resistance to fermented intermediate of butanol and butanol producibility. USE:Used for production of butanol. PREPARATION:For instance, Clostridium pasteurianum DSM 525 stock as a mother stock of genus Clostridium bacterium is anaerobically cultured in a medium in a nitrogen atmosphere at 37 deg.C for 12hr and collected by centrifuge, then subjected to mutation treatment with adding mutagen such as N-methyl-N'- nitro-N-nitrosoguanidine, thus resultant mutant is cultured in a medium containing crotonyl alcohol to afford a mutant of genus Clostridium bacterium having colony of crotonyl alcohol-resistant stock and butanol producibility.