| 摘要 |
PURPOSE: A transformed E.coli containing a vector pTC101 is provided. Also, a production method of tagatose using the same is provided which produces tagatose from galactose by artificially expressing the gene of arabinose isomerase. The method is cost effective in producing tagatose by using low cost of galactose instead of galactitol. CONSTITUTION: A transformed E.coli JM105/pTC101(KCTC-0603BP) is characterized by containing a vector pTC101, of which promoter binds with a gene(araA) of L-arabinose isomerase. The gene(araA) is derived from E.coli, Bacillus, Salmonella, Enterobacter, Klebsiella, Pseudomonas, Lactobacillus, Zymononas, Gluconobacter, Rhyzobium, Acetobacter, Rhodobacter, and Agrobacterium. The transformed E.coli JM105/pTC101(KCTC-0603BP), which produces tagatose, is prepared by the following steps of: a)PCR cloning arabinose isomerase from E.coli; b)inserting a gene(araA) into a vector pKK223-3 to construct a recombinant vector pTC101; c)introducing the vector pTC101 into E/coli JM105 to prepare a transformed E.coli JM105/pTC101(KCTC-0603BP) that produces tagatose. Tagatose is prepared by culturing the transformed E.coli JM105/pTC101(KCTC-0603BP) in a fermentation medium, which contains 10-30g/l of galactose, 7-13g/l of yeast extract, 5-7g/l of potassium monohydrogen phosphate, 2-4 g/l of potassiumdihydrogen phosphate and ammonium chloride. Consequently, galactose is changed into tagatose by expression of arabinose isomerase. |
