LIQUEFACTION OF BONE MATRIX

摘要

The invention relates to methods of preparing a bone matrix solution, a bone matrix implant, and variants thereof. The invention also relates to methods of cell culture using the same. The invention further relates to bone matrix scaffolds comprising one or more bone matrix nanofibers, methods of preparing, and methods of use thereof. The invention also relates to methods of culturing cells and promoting differentiation of stem cells using the same.

主权项

1. A bone matrix implant produced by a method comprising the steps of:
a) preparing a bone matrix solution by dissolving bone in an amount of an anhydrous solvent comprising (i) hexafluoroisopropanol (HFP) or 2,2,2-trifluoroethanol (TFE) and (ii) at least about 5 wt. % trifluoroacetic acid (TEA) sufficient to form the bone matrix solution at a temperature lower than about 25° C.; b) exposing the bone matrix solution to conditions sufficient to evaporate a portion of the solvent to form a colloidal gel; c) coating a surface of an implant with the colloidal gel; and d) exposing the coated surface to conditions sufficient to further evaporate an amount of solvent.