| 摘要 |
<p>A method and kit for calibrating a flow cytometer or fluorescence microscope and for quantitating binding proteins, such as antibodies. Populations of microbeads are provided which are in equilibrium with a saturating amount of fluorescently-labeled protein. The microbeads may have an incorporated fluorescent dye which has a strong signal in a selected channel of a flow cytometer, but no signal in other channels. The kit also includes a software product for determination of selected performance parameters for quality control prior to determining the protein-binding capacity of samples.</p> |
