发明名称 |
ALPHAVIRUS PARTICLES AND METHODS FOR PREPARATION |
摘要 |
<p>Provided herein are methods for producing alphavirus replicon particles in high yield; replicon RNAs are electroporated into permissive cells, where the cells are at a relatively high density, together with at least one helper nucleic acid providing the necessary functions for packaging. After a growth period in appropriate medium, alphavirus replicon particles are harvested from the surfaces of the cells in which they were produced using a salt wash in which the salt concentration is from about 0.2 to about 5 M sodium chloride, calcium chloride, magnesium chloride, potassium chloride, ammonium acetate, ammonium bicarbonate, among others. After dilution, if necessary, the particles can be purified by a suitable chromatographic technique.</p> |
申请公布号 |
EP1590451(A4) |
申请公布日期 |
2007.05.02 |
申请号 |
EP20030813433 |
申请日期 |
2003.12.12 |
申请人 |
ALPHAVAX, INC. |
发明人 |
SMITH, JONATHAN, F.;KAMRUD, KURT;DRYGA, SERGEY;ALTERSON, HAROLD;RAYNER, JON;ALTERSON, KIM;MAUGHAN, MAUREEN, F. |
分类号 |
A61K48/00;C12N7/01;C12N7/04;C12N15/86;(IPC1-7):C12N7/02 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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