摘要 |
The invention is based on the finding that furin belongs to a family of endoproteolytically active enzymes and relates to a process for the in vitro cleavage of a protein by treating the protein in the presence of Ca<2><+> ions with furin or furin-like enzyme, or an endoproteolytically active fragment, derative or fusion protein of furin or furin-like enzyme. The invention can be used for the (micro)biological production of a protein by culturing genetically engineered cells expressing a proform of the protein as well as furin or a furin-like enzyme and isolating the protein formed. The invention also relates to a pharmaceutical composition comprising one or more pharmaceutically acceptable carriers, diluents or adjuvants, as well as an endoproteolytically active amount of furin or a furin-like enzyme, or a fragment or derivative of furin or furin-like enzyme having an endoproteolytic activity. <MATH> |
申请人 |
KATHOLIEKE UNIVERSITEIT LEUVEN |
发明人 |
VAN DE VEN, WILLEM, JAN, MARIE;VAN DEN OUWELAND, ANNA, MARIA, WILHELM;VAN DUIJNHOVEN, JOHANNES, LAMBERTUS;ROEBROEK, ANTONIUS, JOHANNES, MARIA;KONING, PIET, NICO, MARIA |